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KMID : 0545120060160091410
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 9 p.1410 ~ p.1415
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Application of Response Surface Methodology for Optimization of Lactic Acid Production Using Date Juice
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Chauhan Kishor
Trivedi Ujjval
Patel K. C.
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Abstract
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Media components, including date juice, sodium acetate, peptone, and K2HPO4, which were screened by Plackett-Burman fractional factorial design, were optimized for lactic acid production from date juice using the response surface method (RSM). Sodium acetate, peptone (p<0.0001), and (p=0.0029) were highly significant in influencing the lactic acid production. Close correlationship between predicted and experimental values was observed. When the optimum values of the parameters obtained through RSM (25.0 g/l date sugar, 15.0 g/l sodium acetate, 19.1 g/l peptone, and 4.7 g/l ) were applied, lactic acid production (22.7 g/l) increased by 50.33%, compared with unoptimized media (15.1 g/l). The subsequent validation experiments confirmed the validity of the statistical model.
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KEYWORD
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Date juice, lactic acid, Lactobacillus sp, RSM
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